From the nine positives, seven had interpretable data from MLST. Blood samples from suspected or clinically diagnosed cases (n = 50) were initially screened via polymerase chain reaction (PCR) targeting 23S rRNA, 16S rRNA ( rrs2), and lip元2 genes. A simplified MLST scheme targeting three loci ( adk, lipL41, mreA) was performed for rapid direct typing of Leptospira in clinical specimens. Unfortunately, molecular typing of Leptospira isolates is not routinely done in most hospital settings. Multilocus sequence typing (MLST) is a method often used for identification of circulating Leptospira species and disease surveillance. Despite the major threat of leptospirosis to public health in the Philippines, its epidemiologic data remain scarce.
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